Translational_Unit

Part:BBa_K3288047:Design

Designed by: Michelle Ahn   Group: iGEM19_SIS_Korea   (2019-10-17)


pOXB20-RBS(Dawn)-signal peptide (NSP4) fused PETase(28-290;S121E/D186H/R280A)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 256
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 69


Design Notes

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Source

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References